Journal: Mucosal immunology

Article Title: Dendritic cells produce TSLP that limits the differentiation of Th17 cells, fosters Treg development, and protects against colitis.

doi: 10.1038/mi.2011.64

Figure Lengend Snippet: Figure 3 Mouse dendritic cells (DCs) express thymic stromal lymphopoietin (TSLP) after toll-like receptor engagement. ( a , b ) BMDCs were activated with 1 g ml − 1 lipopolysaccharide (LPS), 10 g ml − 1 lipotheicoic acid (LTA), 1 mol l − 1 — C — phosphate — G — (CpG), or 0.1 g ml − 1 flagellin for 3 h. Tslp gene expression was assayed in triplicate by quantitative real-time PCR. Each sample was normalized to Tbp expression. Results represent means ± s.e.m. Data are representative from one of two independent experiments. * P < 0.05, * * P < 0.01, * * * P < 0.001. ( c ) BMDCs were stimulated 3 h with LPS (1 g ml − 1 ) and for 4 h with PMA / ionomicine. Splenic CD11c + DCs were isolated from mice 24 h after intravenous injection with LPS. TSLP was immunoprecipitated from total protein extracts. TSLP expression was detected by western blotting. Protein bands were quantified by densitometry and expressed relative to the untreated sample as arbitrary units. rTSLP, recombinant TSLP; NT, not treated; WT, wild-type.

Article Snippet: In some cultures, different concentrations of recombinant mouse TSLP (R & D Systems) were added (0.2, 1, 5, 10, and 20 ng ml − 1 ).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Expressing, Isolation, Injection, Immunoprecipitation, Western Blot, Recombinant

Journal: Mucosal immunology

Article Title: Dendritic cells produce TSLP that limits the differentiation of Th17 cells, fosters Treg development, and protects against colitis.

doi: 10.1038/mi.2011.64

Figure Lengend Snippet: Figure 4 Dendritic cell-derived thymic stromal lymphopoietin (TSLP) controls Th17 and Treg cell differentiation. 2 × 10 4 BMDCs from C57BL / 6J mice were activated or not with lipopolysaccharide (LPS). After washing, cells were cocultured with 10 5 CD4 + CD25 − T cells from spleens of wild-type (WT) or TSLPR − / − BALB / c mice for 6 days. ( a ) Intracellular cytokine staining was carried out after 6 days of coculture. Left, representative dot plots showing percentages of interferon (IFN)- + and interleukin (IL)-17 + T cells in the CD4 + gate. Right, frequencies of CD4 + IFN- + and CD4 + IL-17 + T cells of three individual experiments are shown. Error bars represent s.e.m. ( b ) After 4 days, cells were stained for CD4 and Foxp3. Percentages of CD4 + Foxp3 + T cells are shown. Error bars represent s.e.m. of triplicate samples. Data are representative from one out of three independent experiments. * P < 0.05, * * P < 0.01, * * * P < 0.001. NS: nonsignificant.

Article Snippet: In some cultures, different concentrations of recombinant mouse TSLP (R & D Systems) were added (0.2, 1, 5, 10, and 20 ng ml − 1 ).

Techniques: Derivative Assay, Cell Differentiation, Staining

Journal: Molecules

Article Title: Lignans from Machilus thunbergii as Thymic Stromal Lymphopoietin Inhibitors

doi: 10.3390/molecules26164804

Figure Lengend Snippet: The inhibitory effects of isolated compounds 1 – 3 on thymic stromal lymphopoietin (TSLP)/TSLP receptor (TSLPR) interaction by ELISA.

Article Snippet: For the pSTAT5 assay measuring TSLP inhibitory activity, recombinant human TSLP was purchased from R&D Systems (Minneapolis, MN, USA) and BD Cytofix/Cytoperm and Alexa Fluor 647 mouse anti-STAT5 (pY694) were purchased from BD Biosciences (San Diego, CA, USA).

Techniques: Isolation, Enzyme-linked Immunosorbent Assay, Inhibition, Control

Journal: Molecules

Article Title: Lignans from Machilus thunbergii as Thymic Stromal Lymphopoietin Inhibitors

doi: 10.3390/molecules26164804

Figure Lengend Snippet: Molecular docking results of isolated compounds 1 – 3 on TSLP/TSLPR interaction.

Article Snippet: For the pSTAT5 assay measuring TSLP inhibitory activity, recombinant human TSLP was purchased from R&D Systems (Minneapolis, MN, USA) and BD Cytofix/Cytoperm and Alexa Fluor 647 mouse anti-STAT5 (pY694) were purchased from BD Biosciences (San Diego, CA, USA).

Techniques: Isolation

Journal: Molecules

Article Title: Lignans from Machilus thunbergii as Thymic Stromal Lymphopoietin Inhibitors

doi: 10.3390/molecules26164804

Figure Lengend Snippet: Molecular docking results of isolated compounds 1 – 3 against human TSLP in complex with TSLPR and interleukin-7 receptor α chain (IL-7Rα): ( A ) three-dimensional (3D) docking image of compound 1 ; ( B ) two-dimensional (2D) docking image of compound 1 ; ( C ) 3D docking image of compound 2 ; ( D ) 2D image picture of compound 2 ; ( E ) 3D docking image of compound 3 ; ( F ) 2D docking picture of compound 3 .

Article Snippet: For the pSTAT5 assay measuring TSLP inhibitory activity, recombinant human TSLP was purchased from R&D Systems (Minneapolis, MN, USA) and BD Cytofix/Cytoperm and Alexa Fluor 647 mouse anti-STAT5 (pY694) were purchased from BD Biosciences (San Diego, CA, USA).

Techniques: Isolation

Journal: iScience

Article Title: Immunometabolic adaptation in monocytes underpins functional changes during pregnancy

doi: 10.1016/j.isci.2024.109779

Figure Lengend Snippet:

Article Snippet: CD163 human antibody (clone GHI/61.1) , Miltenyi , Cat#130-123-249; RRID: AB_2819455.

Techniques: Recombinant, Adhesive, Sequencing, Modification, DC Protein Assay, Staining, Imaging, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Gene Expression, Software, Saline